| Nephropathy | 1. With a single injection of streptozotocin 65 mg/kg, ip in rats, early diabetic nephropathy was produced and revealed as an increased microalbuminuria, elevated creatinine and urea in serum, associated with upregulation of mRNA and protein of DPH oxidase p22phox, p47phox, and p67phox and ET(A), upregulated PKR-like eukaryotic initiation factor 2alpha kinase (PERK), and downregulated Cx43 in the renal tissue. 2. In an experiment using a short interfering R targeting NCALD, we found that silencing of the NCALD led to a considerable enhancement of cell migration, accompanied by a significant reduction in E-cadherin expression, and by an elevation of alpha smooth muscle actin expression in cultured renal proximal tubular epithelial cells. We also identified the association of the landmark SNP with the progression of diabetic nephropathy in a 8-year prospective study (A vs. G, P = 0.03, odds ratio = 1.91, 95% CI 1.07-3. 42). These suggest that the NCALD gene is a likely candidate for conferring susceptibility to diabetic nephropathy. 3. NCALD gene is a likely candidate for conferring susceptibility to diabetic nephropathy. | 21267711 |
